SMAD4 基因敲除细胞 (HCT 116)


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SMAD4 基因敲除细胞 (HCT 116)

SMAD4 Knockout cell line (HCT 116) 订购

货号YKO-H822

价格(元)-

规格 1*10^6

说明书

采用独家红棉系统(www.rc-crispr.com)设计sgRNA片段敲除方案,将两条sgRNA构建到CRISPR-U™载体上,通过电转法/病毒法将载体转染进细胞中,使用极限稀释法制备单克隆,并利用EZ-editor™单克隆基因型鉴定试剂盒(货号:YK-MV-1000)对单克隆进行核酸裂解及PCR扩增,再通过Sanger测序验证基因型,复检合格后进行扩增和冻存。

细胞基本信息

产品货号 YKO-H822
细胞名称 SMAD4 Knockout cell line (HCT 116) 中文名称 人结肠癌细胞
细胞形态 上皮样, 贴壁细胞 传代比例 1:3~1:4
培养体系 90%McCOY's 5A+10% FBS
冻存液体系 50%McCOY's 5A+40% FBS+10%DMSO
特殊备注

STR鉴定

基因信息

基因名
SMAD4
基因ID
4089
全名
SMAD family member 4
别称
DPC4, JIP, MADH4, MYHRS
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

敲除信息

敲除区域: E2 (参考转录本 SMAD4-201)
鉴定方法: PCR+Sanger 测序

构建方法描述

采用独家红棉系统(www.rc-crispr.com)设计sgRNA片段敲除方案,将两条sgRNA构建到CRISPR-U™载体上,通过电转法/病毒法将载体转染进细胞中,使用极限稀释法制备单克隆,并利用EZ-editor™单克隆基因型鉴定试剂盒(货号:YK-MV-1000)对单克隆进行核酸裂解及PCR扩增,再通过Sanger测序验证基因型,复检合格后进行扩增和冻存。
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