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PTGS2 基因敲除细胞 (A549)

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PTGS2 Knockout cell line (A549) 订购

货号：YKO-H756

价格(元)：-

规格： 1*10^6

说明书：

采用独家红棉系统（www.rc-crispr.com）设计sgRNA片段敲除方案，将两条sgRNA构建到CRISPR-U™载体上，通过电转法/病毒法将载体转染进细胞中，使用极限稀释法制备单克隆，并利用EZ-editor™单克隆基因型鉴定试剂盒（货号：YK-MV-1000）对单克隆进行核酸裂解及PCR扩增，再通过Sanger测序验证基因型，复检合格后进行扩增和冻存。

产品详情推荐搭配

细胞基本信息

产品货号	YKO-H756
细胞名称	PTGS2 Knockout cell line (A549)	中文名称	人非小细胞肺癌细胞
细胞形态	上皮样, 贴壁细胞	传代比例	1:3~1:5
培养体系	90% F12K+10% FBS
冻存液体系	50%F12K+40%FBS+10%DMSO
特殊备注

STR鉴定

基因信息

基因名

PTGS2

基因ID

5743

全名

prostaglandin-endoperoxide synthase 2

别称

COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.