ADAM12 基因敲除细胞(HCT116)_源井生物【官网】

ADAM12 基因敲除细胞(HCT116)

当前位置：首页 > EZ-editor™系列产品 > KO细胞系 > ADAM12 Knockout cell line(HCT116)

ADAM12 Knockout cell line(HCT116) 订购

货号：YKO-H612

价格(元)：-

规格： 1*10^6

说明书：

采用独家红棉系统（www.rc-crispr.com）设计sgRNA片段敲除方案，将两条sgRNA构建到CRISPR-U™载体上，通过电转法/病毒法将载体转染进细胞中，使用极限稀释法制备单克隆，并利用EZ-editor™单克隆基因型鉴定试剂盒（货号：YK-MV-1000）对单克隆进行核酸裂解及PCR扩增，再通过Sanger测序验证基因型，复检合格后进行扩增和冻存。

产品详情推荐搭配

细胞基本信息

产品货号	YKO-H612
细胞名称	ADAM12 Knockout cell line(HCT116)	中文名称	人结肠癌细胞
细胞形态	上皮样, 贴壁细胞	传代比例	1:3~1:4
培养体系	90%McCOY's 5A+10% FBS
冻存液体系	50%McCOY's 5A+40% FBS+10%DMSO
特殊备注

STR鉴定

基因信息

基因名

ADAM12

基因ID

8038

全名

ADAM metallopeptidase domain 12

别称

ADAM12-OT1, CAR10, MCMP, MCMPMltna, MLTN, MLTNA

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form.